The only difference in the routes is north of Linden Place, where the Q34 diverges. Local stops are served by the regular Q25 and the Q34. Between the Whitestone Expressway and Jamaica, the Q25 employs limited-stop service, making intermittent stops primarily at major intersections and points of interest. This terminal is shared with the parallel Q65 route, which serves 164th Street.

The routes proceed south to Jamaica Avenue, then west to Sutphin Boulevard, terminating at Sutphin Boulevard and 94th Avenue underneath the Jamaica station for the LIRR and AirTrain JFK. It then turns southeast onto Kissena Boulevard, running the entire distance of the street between Main Street and Parsons Boulevard, and then turns south via Parsons Boulevard. The LIRR Port Washington Branch also stops here, at the Flushing–Main Street station. The route passes the Flushing–Main Street terminal of the IRT Flushing Line ( 7 and trains), where over a dozen bus lines terminate. It then turns west onto Northern Boulevard, and then south onto Main Street in Downtown Flushing (which hosts Flushing Chinatown). The bus then travels east and south via 127th Street, Ulmer Place, and Linden Place, before it merges with the Q34 at 32nd Avenue. The Q25's northern terminal is at Poppenhusen Avenue and 119th Street in College Point. A Jamaica Station-bound Q34 bus on Parsons Boulevard in Jamaica.

Mta buses schedule